Biomedical Engineering Reference
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Fig. 2 Corticosteroid recovery by SPE extraction as a function of solvent composition. Solvents
used in the Wash-2 step were composed of 0.1 % formic acid and the indicated solvent composi-
tion. Corticosteroid recovery vs. solvent composition is shown for the following conditions:
( a ) SPE cartridges were washed respectively with a range of 20-65 % methanol and eluted with
100 % methanol; ( b ) SPE cartridges were washed with 5 % methanol and eluted respectively with
60-100 % methanol (Reproduced with permission from American Chemical Society)
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