Chondroitin Sulfate Surface Engineered Docetaxel-Loaded Liposomes for Tumor Targeting: Design, Development, and Characterization - Proceedings of All India Seminar on Biomedical Engineering 2012 (AISOBE-2012) - page 77

Fig. 3 Organ distribution

studies

Biodistribution Studies

To determine the distribution of liposomal formulation in EAC bearing mice, the
animals were divided into three groups (n = 5); administration of drug was done
through the tail vein. The mice of first group served as control. The second group
was injected with Docetaxel (10 mg/kg), and the third group was treated with
Docetaxed-loaded liposomes (eq 10 mg/kg). After 12 h of administration the
animals were sacrificed and the organs were subjected to estimation of docetaxel
spectrophotometrically.

Results and Discussion

The evaluated characters of liposomal formulation are summarized in Table 1 .In
general, the liposomes were spherical in shape and average particle was in the
range of 15-25 nm (Fig. 1 ). As shown in Fig. 2 , after initial burst within 3 h, the
liposomes effectively released drug which sustained for a period of 48 h.
Biodistribution studies revealed that liposomal docetaxel concentrated more in
tumor as compared to free drug (Fig. 3 ). Animals treated with liposomal docetaxel
survived 29 % more (p \ 0.01) as compared to docetaxel alone. Thus, chondritin
linked docetaxel liposomes demonstrated efficacy in terms of improvement in
quality of life of experimental animals.

Conclusion

The ultimate goal of anticancer chemotherapy is either to destroy or to limit the
growth of cancer cells. Use of chondroitin sulfate-coupled liposomes for effective
delivery of docetaxel is an innovative approach. This concept is advantageous in