Biomedical Engineering Reference
In-Depth Information
others argue for a parallel architecture in which each of these modules acts
directly on the readout modules. In the series model, directionality comes
from expression gradients, and while this could explain a variety of obser-
vations, it has shortcomings that must be addressed. In contrast, the parallel
model requires that the core module acquire directionality via another, as yet
undescribed, mechanism.
Intheupcomingdecade,weexpecttoseeanevenmoreintegrativeandin-
teractive approach between experimental approaches including genetic, cell bi-
ological, and biochemical methods, and increasingly sophisticated mathematical
modeling techniques. Increasingly, precise understanding will allow us to devise
sophisticated genetic manipulations
in vivo
,whichwillaimtoisolateandtesta
specific process that contributes to the polarization mechanism. More powerful
experimental methods will begin to yield a much more detailed understanding
of each molecular pathway and specific protein interaction, and ever more so-
phisticated modeling methods will contextualize their contributions to the PCP
protein localization process and the eventual asymmetric outcome.
ACKNOWLEDGMENTS
Work in the Axelrod lab is supported by grants from NIH/NIGMS. We thank Dr. Yi Guo
for her artistic input and assistance preparing the figures.
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