Tailor-Made and Biodegradable Vascular Scaffolds - Microsurgery: Advances, Simulations, and Applications - page 279

104 ° C), sorbitanmonooleate (Span 80, surfactant) were purchased
from Sigma (St. Louis, MO). Mineral oil and hexane were from Wako
Pure Chemical (Japan). Ferrite particles (average size: 5 μ m) were
kindly donated from Toda Kogyo (Japan). Neodymium magnets were
purchased from NeoMag (Japan).
HUVECs and HuMedia EG2 were purchased from Kurabo (Japan).
D-PBS, carbonic acid buffer, HEPES were purchased from Wako Pure
Chemical. 0.5% type I collagen solution and SYTO 13 were purchased
from Koken (Japan) and Invitrogen (USA), respectively.
Magnetic sugar particles (MSPs) as particulates for leaching were
prepared by an emulsion technique. Figure 8.13 shows the fabrication
process of MSPs. Typically, 60 g of -fructose were melted at 120 ° C
until clear yellow liquid was obtained. Then 3 g of ferrite particles
was added in the solution to append magnetism. The brown-colored
liqueied fructose with ferrite particles was emulsiied into 50
mL mineral oil with 1.3 mL Span 80 at 120 ° C under stirring. The
suspension was cooled down using an ice-bath with effective stirring
to solidify MSPs. After discarding the mineral oil, MSPs were washed
in hexane to remove mineral oil and surfactant completely. The
diameter of MSPs was controlled to desired range by using sieves
in hexane. MSPs were stored in hexane at low temperature (<10 ° C)
to prevent humidity and deformation. In this report, we controlled
the size in the range of 53-106 μ m and 250-500 μ m to demonstrate
the controllability of diameter. In addition, we controlled the size
in the range of 150-500 μ m (MSPs 1), 300-500 μ m (MSPs 2) and
425-500 μ m (MSPs 3) to demonstrate the controllability of standard
deviation in diameter.

Fe 3 O 4 particles

MSPs

Emulsification Sieving

Liquid fructose Oil+Surfactant

b) c) d)

a)

Figure 8.13 Fabrication process of magnetic sugar particles (MSPs) with
controlled diameter.

Owing to the activity of surfactant, liqueied -fructose containing
ferrite particles formed emulsion with mineral oil at 120 ° C. After the
emulsiication, MSPs were successfully solidiied with little damage
on their spherical shape. Figure 8.14 shows an optical microscope