Biomedical Engineering Reference
In-Depth Information
6 nm
1000 nm
Monovalent display
Fv
scFv
Fab
Polyvalent display
IgG antibody
Specific, high affinity
C
Propagate
and repeat
Specific, low affinity
Elute
Human monoclonal
antibody
5-6 cycles
Non-specific
Wash unbound and low affinity
Figure 9.5
Schematic representation of antibody phage display methodology. (A) Antibody structures and their major fragments. (B)
Structure of a filamentous phage with monovalent and polyvalent antibody display. (C) General scheme for the selection of human
monoclonal antibodies.
 
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