Biomedical Engineering Reference
In-Depth Information
London APP Mice
(a)
50
40
***
30
***
20
WT
Latency
10
0
Control
CA074Me
E64d
London APP Mice
(b)
350
300
250
**
200
***
150
100
50
0
Control
CA074Me
E64d
Figure 6.6
Inhibitors CA074Me or E64d improve memory deficit in London APP
mice, which express the WT b-secretase site sequence, as assessed by the
Morris water maze test. (a) Improved memory was illustrated by shor-
tened latency times for CA074Me and E64d treated animals. Memory
function was assessed in the Morris water maze test after administration of
CA074Me or E64d by icv administration.
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CA074Me is a prodrug form
of CA074 (conversion to CA074 by cellular esterases), a specific inhibitor
of cathepsin B. E64d is a prodrug form of E64c (conversion to E64c by
cellular esterases). The latency period measures the time it takes the animal
to swim to a submerged platform after training, with shorter times
reflecting improved memory. Results are displayed as the mean latency
period (seconds)
SD with statistical significance indicated
(***p
o
0.0001, Student's t-test). The latency time for wild-type (WT),
normal mice is illustrated by the dotted line. Results show that treatment
with the inhibitors improves the memory deficits towards normal memory
function of WT mice. (b) Improved memory shown by shorter distances
traveled for CA074Me and E64d treated animals. The distance traveled to
the platform is a measure of memory in the Morris water maze test, with
shorter distances reflecting improved memory. Results are shown as the
mean distance traveled (cm)
SD, with statistical significance indicated
(***p
o
0.0001, **p
o
0.005, Student's t-test).
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