Biomedical Engineering Reference
In-Depth Information
43.
A chemical sensor is used to measure the pH of a dye with an absorbance spectrum shown in
Figure 10.45. Assume that the absorbance of each form of the dye is linearly related to its pH.
Devise a method to measure the pH of the dye.
1.0
pH=8.0
0.8
0.6
0.4
pH=5.0
0.2
400
500
600
700
Wavelength [nm]
FIGURE 10.45
Optical absorbance spectra of a dye in its acid (pH ¼ 5.0) and base (pH ¼ 8.0) forms.
44.
Explain the difference between absorption-based and fluorescence-based measurements.
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