Biomedical Engineering Reference
In-Depth Information
Normalized CFU-GM
1.75
C
1.5
1.25
1
.75
.5
.25
0
-.25
-.5
1
2
550000
3
450000
4
350000
Medium Exchange
Interval (days)
5
250000
Inoculum Density
6
150000
7
50000
Normalized CFU-GM
Expansion
1.5
D
1.25
1
.75
.5
.25
0
1
2
550000
3
450000
4
350000
Medium Exchange
Interval (days)
5
250000
Inoculum Density
6
150000
7
50000
FIGURE 6.35
The performance of a bone marrow culture system over a wide range of inoculum densities and
medium exchange intervals. Note that the variables interact. The performance (on the
axis) in the top panel is
the total number of progenitor cells (CFU-GM) per unit area, whereas in the bottom panel the expansion ratio
is depicted (number out divided by number in). Note that the two measures are optimized under vastly different
conditions.
z-
From [8].
