Biomedical Engineering Reference
In-Depth Information
CHAPTER 16
Biosensors based on immobilization of
biomolecules in sol-gel matrices
Vivek Babu Kandimalla, Vijay Shyam Tripathi, and Huangxian Ju
16.1 Introduction
16.2 Sol-gel
16.2.1
Sol-gel chemistry and matrix characteristics
16.2.2
Progress in sol-gel process
16.2.3
Advantages and disadvantages
16.2.4
Porosity and dynamics of proteins in sol-gel
16.2.5
Interactions and stability of biomolecules in sol-gel
16.2.6
Improvement of biocompatibility and conductivity of sol-gels
16.3 Applications of sol-gel entrapped bioactive molecules
16.3.1
Enzyme-based biosensors
16.3.1.1
Biosensor applications of enzymes
16.3.1.2
Carbon-ceramic composite electrodes (CCEs)
16.3.1.3
Electrode surface coatings
16.3.1.4
Optical biosensors
16.3.1.5
Electrochemical biosensors
16.3.2
Photoactive proteins-based biosensors
16.3.3
Immunosensors
16.3.4
Immunoaffi nity columns
16.4 Whole-cell encapsulation in sol-gels and their applications
16.4.1
Microbial cells
16.4.2
Plant and animal cells
16.5 Conclusions
16.6 Acknowledgments
16.7 References
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