Biomedical Engineering Reference
In-Depth Information
10
2
10
4
10
6
10
8
10
9
10
8
10
7
10
6
10
5
10
4
10
3
10
2
10
1
[H
2
O
2
], M
FIGURE 13.6
Calibration plot for hydrogen peroxide detection in fl ow-injection mode with nano-struc-
tured Prussian blue as a detector; Prussian blue electrodeposited through sol template based on the vinyltri-
ethoxysilane, operating potential 50 mV, phosphate buffer pH 6.0
0.1 M KCl, fl ow rate 0.7 ml/min.
blue can be considered to be an ideal electrocatalyst for hydrogen peroxide reduction.
The above examples illustrate the performance characteristics of Prussian blue-based
transducers, which offer exceptional benefi ts to electroanalysis.
13.5 BIOSENSORS BASED ON TRANSITION METAL
HEXACYANOFERRATES
13.5.1 Transducing principles for oxidase-based biosensors
More than 90% of commercially available enzyme-based biosensors and analytical kits
contain oxidases as terminal enzymes responsible for generation of analytical signal.
These enzymes catalyze oxidation of specifi c analyte with molecular oxygen produc-
ing hydrogen peroxide according to the reaction:
Oxidase
Oxidized
Analyte
Analyte
(4)
O
2
H
2
O
2
Among different approaches providing operation of the oxidase-based biosensors,
the detection of hydrogen peroxide production was found to be the most progressive
one, allowing detection of low levels of analytes [107]. However, the detection of H
2
O
2
has to be carried out at low potentials in order to reduce the interference of easily oxi-
dizable compounds [110].
Search WWH ::
Custom Search