Biomedical Engineering Reference
In-Depth Information
Coord
First
derivative
Second
derivative
FIGURE 11.21
Spotfi nding using adaptive circle segmentation method.
as a foreground. On the other hand, if the pixel value is smaller than the predetermined
value, the pixel is classifi ed as the background.
3. Information extraction [29]
After determining the shape, size, and address of each spot in the microarray, the next
step is to calculate the foreground and the background intensities. The foreground
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