Biomedical Engineering Reference
In-Depth Information
test sample will result in red spots. Yellow spots occur when the gene hybridizes with
both control and test samples.
Figure 11.15 (see Plate 5 for color version) shows ideal microarray spots. However,
such ideal round spots are in fact impossible to achieve. As suggested by Yang et al.
[28], the imperfect spot size and shape occurs during the hybridization and printing
process and is also due to the conditions of the slide surface, making it more tedious
to extract the raw data. Some of the imperfect microarray spots appear in the form of
a “comet tail”, as shown in Fig. 11.16 (see Plate 6 for color version), which is due to
FIGURE 11.15
Ideal microarray spots (see Plate 5 for color version).
FIGURE 11.16
Comet tails (see Plate 6 for color version).
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