Biomedical Engineering Reference
In-Depth Information
TSA Method
1. Standard ABC Method
P
B
P
B
A
B
P
B
B
Streptavidin-biotin HRP
Complex (15 mins)
B
A
B
P
Biotinylated
secondary antibody
(15 mins)
B
B
Primary antibody
(15 mins)
Antigen
Antigen
FIGURE 11.12
TSA method using the conventional ABC method, together with pretreatment proce-
dures and optimal antigen retrieval. Source: www.hmds.org.uk/ histology.html
TSA Method
2. Biotinylated Tyramine
Amplification
P
Inactive biotinyl
tyramine
B
P
B
A
B
P
B
B
B
Reactive biotin
B
A
B
P
B
B
B
BB
B
B
FIGURE 11.13
Formation of reactive biotinylated tyramide through the catalytic reaction HRP and
hydrogen peroxide catalyses. Source: www.hmds.org.uk/histology.html
is specialized for acquiring microarray fl uorescent images for arrays detection. The
three most widely used scanner brands from the ABRF survey 2001 are Packard/GSI
Lumonics (34%), Axon (24%), and Affymetrix/GMS (13%). There are many factors
that determine the performance of a microarray scanner, like sensitivity, resolution,
and dynamic range. In general, there are three major considerations in technologies
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