Biomedical Engineering Reference
In-Depth Information
TABLE 7.1
Summary of fi eld-effect-based DNA sensors reported in literature
Hybridization
Sensor type
Probe ssDNA; density
Electrolyte
Target cDNA
Sensor signal
time
RE
Ref.
FET; EIS p-Si-SiO
2
oligo(dT
20
); poly(dT)
50 mM NaCl;
oligo(dA
18
);
∆
V
fb
100 mV;
4 h
Ag/AgCl
[11]
(10 nm, silanization
(1000 bases); brominated
10 mM Tris-HCl,
poly(dA)
∆
V
th
120 mV at
with APTES)
oligonucleotides
pH 7.1
(1000 bases); calf
2
µ
g/ml target DNA
thymus DNA
n-channel FET;
17-mer ssDNA;
25 mM
17-mer cDNA
∆
V
th
11 mV
15 h
Ag/AgCl
[27]
Si-SiO
2
-Si
3
N
4
;
1.7
10
8
ssDNA/cm
2
;
Na
2
HPO
4
amino-silanized
glutaraldehyde as cross-
pH 6.86
linking agent
EIS; Si-SiO
2
17-mer ssDNA;
25 mM Na
2
HPO
4
17-mer cDNA
∆
V
fb
20 mV
12 h
Ag/AgCl
[45]
(30 nm)-Si
3
N
4
3.7
10
8
ssDNA/cm
2
;
pH 6.86
(50 nm); amino-
glutaraldehyde as cross-
silanized
linking agent
n-Si-SiO
2
(2 nm)-
12-mer ssDNA; adsorption
23 mM
12-mer cDNA
∆
V
fb
3 mV;
10-15 min
Ag/AgCl
[43]
poly-L-lysine
5
10
13
ssDNA/cm
2
Au-gate n-channel
thiolated 15- or
1 M NaCl
15-mer cDNA
∆
V
th
10 mV
1000 s
Ag wire
[13]
depletion FET;
25-mer ssDNA
pH 7.4
25-mer cDNA
∆
V
th
3 mV
p-Si-SiO
2
(63 nm)-
Si
3
N
4
(30 nm)-Au
Ta
2
O
5
-gate FET;
amino-modifi ed 21-mer
0.5 M MgCl
2
21-mer cDNA
∆
V
th
10 mV
overnight
Ag/AgCl
[24]
silanized
ssDNA; 3.6
10
5
ssDNA/cm
2
n-channel FET;
20-mer ssDNA;
phosphate buffer,
detection of probe
∆
V
th
1.9 V
3.5 h
without RE
[18]
p-Si-SiO
2
(50 nm)
adsorption
pH 7
ssDNA
p-channel FET;
adsorption
4
10
11
1 mM NaCl
PolyA, 20 or
∆
V
th
5 mV
1 h
Ag/AgCl
[14]
n-Si-SiO
2
(8-12 nm);
ssDNA/cm
2
45 bases
liquid-
silanization with
junction
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