Biomedical Engineering Reference
In-Depth Information
carbon fiber 10 m in diameter
500 m
glass capillary
25 ~ 50 mm
epoxy resin
Cu wire
(a)
…
Au nanoparticle
NH
3
electrodeposition
cysteine
COO
S
CH
2
CH
NH
3
COO
S
CH
2
CH
CFME
…
…
NH
3
COO
S
CH
2
CH
SOD
NH
3
SOD
COO
S
CH
2
CH
(b)
…
SCHEME 4
(a) Carbon fi ber microelectrode, and (b) the process of electrode modifi cation. (Reprinted
from [158], with permission from Elsevier.)
(about 500
m) with a scalpel under a microscope. Prior to electrochemical activation,
the fabricated carbon fi ber microelectrodes were ultrasonically washed with acetone,
3.0 M HNO
3
, 1.0 M NaOH, and distilled water sequentially. The electrode pretreatment
was performed in a 0.50 M sulfuric acid solution by applying a constant potential at the
electrodes, fi rst,
µ
1.0 V for 10 s, and then the electrodes were
treated by using cyclic voltammetry in the potential range from 0.0 to
2.0 V for 30 s, then
1.0 V at a scan
rate of 100 mV/s until a stable cyclic voltammogram was obtained [159, 160].
The Au-NPs were electrodeposited on the carbon fi ber microelectrodes from 0.5 M
H
2
SO
4
solution containing 1.0 mM Na[AuCl
4
] by applying a potential step from 1.1 V
to 0 V for 30 s. Cysteine-modifi ed Au-NPs-electrodeposited CFMEs were prepared by
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