New trends in ion-selective electrodes - Electrochemical Sensors, Biosensors and Their Biomedical Applications - page 105

Reference electrode

Reservoir

Physiological medium

Living cells

Insulation layer

Si

Ion selective membrabne

Pt electrodes

Silicon substrate

Pyrex

Pyrex substrate

Electrode 1

Electrode 2

(a)

(b)

FIGURE 4.14 Schematic cross-section of the platform for in-vitro intracellular recording of ion con-
centration, designed for hepatocyte cell culture (a) and ESEM pictures of a micropipette (tilted at 75º) fi lled
with the membrane cocktail. (From [102].)