Biomedical Engineering Reference
In-Depth Information
10
7
A
B
off
200
10
6
100
10
5
10
4
0
on
0
2
4
6
8
10
10
-1
10
0
10
1
10
2
10
3
10
4
10
5
10
6
t
/s
f
/ Hz
10
7
D
C
2
10
6
off
1
10
5
2
M
10
4
0
0
M
on
0
2
4
6
8
10
10
-1
10
0
10
1
10
2
10
3
10
4
10
5
10
6
t
/s
f
/ Hz
Figure 21. A/C) Photocurrent measurements on pore-spanning membranes incubat-
ed with bR-doped liposomes upon illumination using a halogen lamp as a light
source and a cut-off-filter of 515 nm. B) (Ƒ) Impedance spectrum 7.5 h after the
addition of DPhPC/DOPC (6:4) LUVs doped with 0.1 mol% octadecylamine. (ż)
Impedance spectrum 12 h after the addition of bR-doped vesicles composed of
DPhPC/DOPC/DPPA (6:3:1) containing 1 mol% bR. Data analysis was performed
using the equivalent circuit depicted in Fig. 5 B with the following results: (Ƒ)
R
el
=
1700 ȍ,
R
m
= 4.3·10
5
ȍ, A = 2.4·10
-8
F s
Į
-1
and
Į
= 0.77; (ż)
R
el
= 1700 ȍ,
R
m
=
2.2·10
4
ȍ, A = 1.7·10
-7
F s
Į
-1
, and
Į
= 0.71. D) (Ƒ) Impedance spectrum 24 h after
the addition of DPhPC/DOPC (6:4) LUVs doped with 0.1 mol% octadecylamine.
(ż) Impedance spectrum 14 h after the addition of bR-containing vesicles. Data
analysis was based on the equivalent circuit depicted in Fig. 7 C with the following
result: (Ƒ)
R
el
= 5890 ȍ,
R
m1
= 1.1·10
5
ȍ,
C
m1
= 0.10·10
-9
F,
R
m2
= 6.1·10
6
ȍ, and
C
m2
= 0.9·10
-9
F; (ż)
R
el
= 2290 ȍ,
R
m1
= 3.1·10
5
ȍ,
C
m1
= 0.15·10
-9
F,
R
m2
= 1.7·10
6
ȍ and
C
m2
= 1.81·10
-9
F. Buffer: 10 mM TRIS, 100 mM KCl, pH 7.4.
bR-containing vesicles hemifused to the pore-suspending mem-
brane. In this case, two populations of bR-molecules contribute to
the monitored light-induced photocurrent. Proteins that span the
bilayer as found in scenario A provide the stationary current, while
bR remaining in hemi-fused liposomes build up a transient current
similar to what has been found for PM-fragments adsorbed to
nano-BMLs. In this case, the equivalent circuit shown in
Fig. 17 A
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