Electrochemical Analysis of Ion Channels and Transporters in Pore-Suspending Membranes - Applications of Electrochemistry and Nanotechnology in Biology and Medicine - page 295

A

6

I / pA

4

2

-150 -100 -50 50 100 150 200

-2

V h / mV

-4

-6

B

8

6

4

2

0

50 100 150 200

G / pS

Figure 11. A) Cx26 current-voltage relationship of averaged current steps ranging
between 0-63 pS (2169 events). The hemichannel shows a linear I - V h -dependence
with a main conductance of (33 ± 3) pS. B) Event-histogram of the observed con-
ductance states of Cx26 in nano-BLMs (2691 events, bin width: 2 pS). The solid
line is the results of fitting three Gaussian distributions to the histogram. Three
main conductance states are assigned to G 1 = (34 ± 8) pS, G 2 = (70 ± 8) pS, and G 3
= (165 ± 19) pS. 69