Electrochemical Analysis of Ion Channels and Transporters in Pore-Suspending Membranes - Applications of Electrochemistry and Nanotechnology in Biology and Medicine - page 289

A

80

60

40

20

0

2.5

B

2.0

1.5

1.0

0.5

0.0

0 100 200 300 400 500 600

G / pS

6

C

5

4

3

2

1

0

100 200 300 400 500 600

G / pS

Figure 10. A) Single channel conductance values
G of gramicidin D in a DPhPC nano-BLM on a
porous alumina substrate with a mean pore diame-
ter of 280 nm. Three different cations with a con-
centration of 500 mM were added to both sides of
the porous substrate. B) Histogram analysis of the
observed conductance states of the 2,2'-
bipyridine-functionalized peptidic ion channel in
nano-BLMs up to 1000 pS (3556 events, bin
width: 5 pS, normalized to all events (4524)) in
the absence of NiCl 2 . C. Histogram analysis of the
conductance states of the 2,2'-bipyridine-
functionalized peptidic ion channel in the pres-
ence of 2.5-5 μM NiCl 2 up to a conductance of
1000 pS (2029 events, bin width: 5 pS, normal-
ized to all events (2189)). The solid lines are the
results of fitting four Gaussian distributions (dot-
ted lines) to the histograms. 58