Biomedical Engineering Reference
In-Depth Information
6
10
5
10
4
10
10
-1
10
0
10
1
10
2
10
3
10
4
10
5
10
6
f
/ Hz
Figure 8. Absolute value of the impedance |
Z
| of a pore-spanning bilayer from
LUVs (DPhPC/DOPC 6:4) on CPEO3-functionalized porous alumina with selec-
tively opened pore-bottoms with
R
ox
= 8.9 Mȍ und
C
ox
= 6.7 nF, previously de-
termined by EIS. The dashed line is the result of fitting the parameters of the
2.6 Mȍ,
C
m,o
= 1.9 nF,
R
m,c
= 0.89 Mȍ and
C
m,c
= 5.8 nF. The solid line is the
Mȍ,
C
a
= 8.2 nF. Buffer: 10 mM TRIS, 100 mM TMA, pH 8.6.
38
the capacitive properties of the membrane itself are obtained, the
overall resistance
R
a
and, after applying Eq. (3),
R
m,c
and
R
m,o
can
be accurately determined in a simple fit routine. This is a prerequi-
site for the analysis of impedance data obtained in the presence of
active ion channels, as further discussed in Section IV.
III. RECONSTITUTION OF PEPTIDES IN NANO-BLMS
As outlined in Section II, nano-BLMs are clearly advantageous
over classical BLMs, as they exhibit similar high membrane re-
sistances, but are at the same time attached to a substrate, which
makes these membranes long-term and mechanically stable. In the
following paragraphs, several examples will be given to demon-
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