Biomedical Engineering Reference
In-Depth Information
A
10
9
R
el
10
7
R
m
C
m
10
5
10
3
B
-90
-60
-30
0
10
-2
10
-1
10
0
10
1
10
2
10
3
10
4
10
5
10
6
f
/ Hz
C
0
-20
-40
-60
-80
0
50
100
150
200
250
t
/ s
Figure 2. Bode representations of the impedance
data [A) Magnitude of the impedance, B) phase
angle between current and voltage] of gold-
covered porous alumina membranes functional-
ized with a DPPTE-monolayer before (Ŷ) and one
day after (Ƒ) the application of DPhPC dissolved
in
n
-decane on the porous alumina substrate to
form a nano-BLM. The solid lines are the results
of the fitting procedure to (Ƒ) using the equivalent
circuit shown in the inset:
C
m
= 11.7 nF,
R
m
= 4.7
Gȍ. Inset: Equivalent circuit composed of a paral-
lel
RC
-element (
R
m
and
C
m
) representing the elec-
trical behaviour of a lipid bilayer in series to an
Ohmic resistance
R
el
that represents the electrolyte
solution and the wire connections.
24
C) Time-
resolved increase in I(10
6
Hz) during the mem-
brane formation process, allowing to follow the
kinetics and success of the thinning process. Elec-
trolyte: 1 M KCl, 1 mM CaCl
2
, pH 6.0.
26
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