Biomedical Engineering Reference
In-Depth Information
Figure 1. A)
Teflon cell that is used for impedance analysis and
voltage clamp experiments on nano-BLMs. The porous alumina
substrate is clamped between two O-rings, which define the active
area. An electrode is placed in each compartment (two-electrode
configuration), which are connected to either an impedance ana-
lyser or the headstage of the patch clamp amplifier. B) Setup used
for electrical measurements on pore-spanning membranes obtained
from vesicle spreading. The porous substrate is located between
the two upper parts of the Teflon chamber by means of sealing
rings. The single pieces of the setup are tightly secured via the
third part of the setup, which serves as the
trans
compartment,
whereas the chamber on top is the
cis
compartment. Platinised
platinum electrodes in the
cis
and
trans
compartment (two-
electrode configuration) serve to detect the signal in impedance
and photocurrent measurements.
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