Biomedical Engineering Reference
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Figure 22. (a) Gold-supported SbpA layer pressed against a perforated plastic sheet,
with a lipid bilayer transferred onto the SbpA layer by the Langmuir-Blodgett tech-
nique. (b) Same as (a), apart from the replacement of the SbpA layer by SUM. (c)
Lipid bilayer supported by SUM and interposed between two bulk aqueous phases.
The side of the SUM with attached the S-layer lattices was turned
toward the chamber. A DPhyPC lipid bilayer on top of the SqbA-
or SUM-coated gold was formed by a modified Langmir-Blodgett
technique. To this end, the chamber was filled with an aqueous
electrolyte above the orifice level, a DPhyPC solution is
n
-hexane
was spread on the electrolyte surface and the organic solvent was
allowed to evaporate. By lowering the electrolyte level below the
orifice, a first lipid monolayer was deposited on the coated gold,
and a second monolayer was deposited by raising again the elec-
trolyte level.
A tetraetherlipid (TEL) was also used in place of DPhyPC.
This is a bipolar, membrane-spanning phospholipid consisting of a
72 member microcycle formed by two glycerol units that are
bridged by two biphytanoyl chains. While in phospholipids the
alkyl chains are connected to the glycerol moieties by ester bonds,
in tetraetherlipids they are connected by four ether bonds, provid-
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