Biomedical Engineering Reference
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Figure 21. Schematic picture of S-layer stabilized solid supported lipid membranes.
(a) S-layer directly recrystallized on gold, with a lipid bilayer on top. (b) Same as
(a), with an additional S-layer recrystallized on top of the lipid bilayer. (c) Thiolat-
ed secondary cell wall polymers (SCWPs) directly bound to gold and interacting
with a S-layer, with a lipid bilayer on top. (d) Same as (c), with an additional S-
layer recrystallized on top of the lipid bilayer. (e) S-layer directly recrystallized on
gold and interacting with lipidated SCWPs, which anchor a lipid bilayer. (f) Same
as (e), with lipidated SCWPs inserted into the distal lipid monolayer and interacting
with an additional S-layer.
The natural tendency of S-layers to interact with membranes
has been exploited to insert them as an intermediate layer between
a lipid bilayer and a substrate, giving rise to the so-called
S-layer
stabilized bilayer lipid membranes
(ssBLMs). Thus, in the case of
bacterial S-layer proteins, it has been demonstrated that protein
domains or functional groups of the S-layer lattice interact via
electrostatic forces with some head groups of lipid molecules. In
addition, the affinity of S-layer proteins for the corresponding
SCWPs, which are recognized as specific binding sites, can be
used to fabricate complex architectures (see
Fig. 21
). Thus, a mon-
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