Electrochemistry of Biomimetic Membranes - Applications of Electrochemistry and Nanotechnology in Biology and Medicine - page 163

The angular position of the minimum of the SPR reflectivity
curves (i.e., the curves of reflectivity versus incident angle; see
Fig. 7b ) is critically dependent on the thickness of the layer ad-
sorbed on the support surface. When macromolecules assemble
Figure 7. (a) Experimental set-up for surface plasmon resonance measurements,
combined with an EIS module for simultaneous SPR and electrochemical meas-
urements on tBLMs. The enlargement shows the solid/solution interface with the
thin Au layer used for surface plasmon excitation, and the tethered lipid bilayer in
contact with the aqueous phase; (b) typical SPR reflectivity curves before and after
the formation of the distal lipid monolayer (on top of the self-assembled proximal
tethered lipid monolayer) by vesicle fusion; (c) kinetics of the fusion process rec-
orded by monitoring the change of reflectivity at a fixed angle of incidence as a
function of time; (d) time dependence of the small-amplitude a.c. voltage used in
EIS measurements and of the resulting a.c. current of equal frequency and different
phase angle. (Reprinted from Ref. 9b with kind permission from Elsevier.)