Biomedical Engineering Reference
In-Depth Information
Figure 4. Top: An array consisting of tetranucleotides with 4 4 = 256 spots. Alt-
hough each spots is depicted only one strand per spot, it contains many copies
of the same oligonucleotide. Bottom: Summary of the SHB experiment. With
treating by a partially digested DNA strand, hybridization occurred in six dif-
ferent spots, colored in black. After identifying the sequence of these spots and
sorting them, the sequence of the sample DNA strand can be reconstructed.
improvement in the methods used. Normally employed methods of
sequencing (direct methods such as gel electrophoresis and py-
rosequencing) determine each consecutive base position in the
DNA chain individually. In contrast, an indirect method assembles
the DNA sequence based on experimental determination of the
oligonucleotide content of the DNA chain. Sequencing by hybrid-
ization (SBH) is a promising indirect method in which sets of oli-
gonucleotides are hybridized under conditions that allow detection
of complementary sequences in the target nucleic acid. 12
Figure 4 shows the procedure of base sequence determination
exemplifying a tetramer DNA sample. An array consisting of 256
 
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