Nanotoxicity - The Nanobiotechnology - page 601

Cerebral cortex Hippocampus CA1 Hippocampus DG

Control Control Control

A A A

B B B

C C C

D D D

Figure 27.1
(See companion CD for color igure.) . Physicochemical. characteristics. of. TiO 2 . determine. its. neurotoxicity.
in vivo .. Morphological. changes. of. neurons. in. the. HE. stained. brain. tissue. sections. of. the. cerebral. cortex. and.
hippocampus.(CA1.and.DG).in.the.mice.after.intranasal.instillation.of.different.TiO 2 .particles.(sample.A.are.
hydrophobic,. micrometer-sized. particles;. sample. B. are. hydrophobic,. nanometer-sized. particles;. C. and. D. are.
hydrophilic,.nanometer-sized.particles,.and.the.structure.of.C.is.needle.like,.D.is.rod.like)..Normal.pyramidal.
cells.showed.round.and.pale.stained.nuclei,.whereas.dying.or.dead.cells.showed.pyknotic.nuclei..The.normal.
structure.of.pyramidal.cells.has.round/oval.and.pale.stained.nuclei.as.well.as.a.clear.nucleolus..Damaged.neu-
rons.have.shrunken.cell.bodies,.deeply.stained.pyknotic.nuclei.with.a.triangular.or.elongated.proile,.a.nucleo-
lus.that.disappeared,.as.well.as.the.widened.gap.between.the.nuclei.and.the.cell.membrane..Arrows.indicate.the.
damaged.neurons..Magniication:.×200..(Reproduced.with.modiication.by. Toxicol Lett ,.207,.Zhang,.L.,.Bai,.R.,.
Li,.B.,.Ge,.C.,.Du,.J.,.Liu,.Y.,.Le.Guyader,.L..et.al.,.Rutile.TiO 2 .particles.exert.size.and.surface.coating.dependent.
retention.and.lesions.on.the.murine.brain,.73-81,.2011..Copyright.2011,.with.permission.from.Elsevier.)

inluenced.their.toxicity,.metabolism.kinetics,.and.biomedical.functions..In.an.in.vitro.
study,.the.SWCNTs,.MWCNT,.and.C 60 .exhibited.quite.different.cytotoxicities.to.alveolar.
macrophages,. although. they. are. associated. with. carbon. materials.. CNT. exposure.
results. in. a. loss. of. the. phagocytic. ability. and. in. ultrastructural. injury. of. alveolar.
macrophages.. The. comparative. toxicities. exhibited. the. following. sequence. order. on. a.