Biomedical Engineering Reference
In-Depth Information
interactions. control. cell. behavior. and. inluence. complex. cellular. processes,. including.
stem-cell.differentiation.and.tissue.organization.(Bettinger.et.al..2009b).
22.3.1 Cell response to Nanoscale Features
Cells.naturally.interact.with.topographical.structures.in.their.surroundings,.often.through.
contact. guidance. phenomena.. Under. the. inluence. of. contact. guidance,. cells. respond. to.
surrounding.structures.on.the.micrometer.and.sub-micrometer.scale.in.vivo.(Wolf.et.al..
2003)..It.also.plays.an.essential.role.in.the.migration.of.individual.cells.or.groups.of.cells.or.
tissue.(Friedl.2004),.and.effects.eficient.organelle.formation,.such.as.axonal.guidance.and.
growth.cone.motility.(Dent.and.Gertler.2003,.Dent.et.al..2003).
As. mentioned. earlier,. nanoscale. features. provide. mechanotransductive. cues. to. cells,.
and. inluence. them. in. many. ways.. One. of. the. most. prominent. cell. responses. toward.
nanotopographic. features. are. morphologic. changes. on. cells,. especially. ibroblasts,.
endothelial. cells,. epithelial. cells,. stem. cells,. and. Schwann. cells. (Hsu. et. al.. 2005).. Cell.
morphology.responds.to.nanogratings.by.simultaneously.aligning.and.elongating.along.
the. direction. of. the. grating. as. shown. in. Figure. 22.3. (Teixeira. et. al.. 2006).. In. addition. to.
(a)
Nano
(b)
5 μm
Flat
5 μm
Figure 22.3
(See companion CD for color igure.)
.Epithelial.cell.responses.toward.nanograting.in.epithelial.cells..Epithelial.
cells. align. and. elongate. along. the. grating. axis,. as. evident. from. luorescent. (a). and. SEM. (b). micrographs..
(Reprinted.from.
J. Cell Sci
.,.116,.Teixeira,.A.I.,.Abrams,.G.A.,.Bertics,.P.J.,.Murphy,.C.J.,.and.Nealey,.P.F.,.Epithelial.
contact.guidance.on.well-deined.micro-.and.nanostructured.substrates,.1881-1892..Copyright.2003,.with.per-
mission.from.Elsevier.)
