Optical Tweezers - The Nanobiotechnology - page 455

(a)

29 pN

0 pN

109 pN

67 pN

(b)

5 μm

(c)

Before dissection After dissection

(d)

Figure 19.10
Optical. cell. assembly. and. surgery.. (a). Various. assembling. patterns. of. live. mouse. stem. cells. by. optical. twee-
zers..(Reproduced.with.permission.from.Leach,.J.,.Howard,.D,.Roberts,.S.,.Gibson,.G.,.Gothard,.D.,.Cooper,.J.,.
Shakesheff,.K.,.Padgett,.M.,.and.Buttery,.L.,.Manipulation.of.live.mouse.embryonic.stem.cells.using.holographic.
optical. tweezers,. J. Mod. Opt .,.56,. 448.. Copyright. 2009,. Taylor. &. Francis. Group.). (b). Deformation. of. red. blood.
cells.in.phosphate.buffer.saline.solution.with.various.trapping.forces..(Reprinted.from. Acta Mater .,.52,.Lim,.C.T.,.
Dao,. M.,. Suresh,. S.,. Sow,. C.H.,. and. Chew,. K.T.,. Large. deformation. of. living. cells. using. laser. traps,. 1837-1845..
Copyright.2004,.with.permission.from.Elsevier.).(c).Induced.cell.fusion.for.a.pair.of.cells.trapped.and.positioned.
by.optical.tweezers..(Steubing,.R.W.,.Cheng,.S.,.Wright,.W.H.,.Numajiri,.Y.,.and.Berns,.M.W.:.Laser.induced.cell.
fusion.in.combination.with.optical.tweezers:.The.laser.cell.fusion.trap.. Cytometry ..1991..12..505-510..Copyright.
Wiley-VCH.Verlag.GmbH.&.Co..KGaA..Reproduced.with.permission.).(d).Dissection.of.cell.cluster.with.ultravio-
let.laser.pulses..The.black.circle.on.the.left.image.indicated.the.dissection.region.while.the.isolated.cell.cluster.is.
shown.on.the.right.image..(Reprinted.with.permission.from.Stuhrmann,.B.,.Jahnke,.H.G.,.Schmidt,.M.,.Hah¨n,.K.,.
Betz,.T.,.Müller,.K.,.Rothemel,.A.,.Kas,.J.,.and.Robitzki,.A.A.,.Versatile.optical.manipulation.system.for.inspection,.
laser.processing,.and.isolation.of.individual.living.cells,. Rev. Sci. Instrum .,.77,.063116..Copyright.2006,.American.
Institute.of.Physics.)