Biomedical Engineering Reference
In-Depth Information
×10
5
(V/m)
10 V
1.8
Objective
lens
1.4
Laser
beam
1.0
in Al
ITO glass substrate
0.6
Silicon
nitride
Amorphous
silicon
R
0.2
σ(
R
)
I
(
R
)
AC
PR
spacer
Cells
17 μm beam
width
ITO glass
R
0
(a)
(b)
t = 0 min
100 μm
t = 1 min
100 μm
100 μm
(c)
(d)
(I)
(II)
24 (μm)
(I)
(II)
Conveyor
Joint
20
16
12
8
4
0
0 5 10 15 20 25
Z position (μm)
10 (μm)
Sorting path
z
Live
cell
Dead
cell
Dynamic wedge
100 μm
30 35 40 45 50
(III)
(IV)
(III)
(IV)
24 μm
24 μm
Dead
cell
10 μm
10 μm
Live
cells
(e)
(f )
Figure 17.7
(See companion CD for color igure.)
.The.optoelectronic.tweezers.(OET)..(a).The.schematic.of.the.OET.structure.
and.its.working.mechanism..The.liquid.medium.is.illed.in.the.gap.between.two.ITO-coated.glass.electrode.plates.
with.the.top.one.coated.with.multiple.featureless.layers.including.a.thin.Al.layer.(to.reduce.the.contact.resistance),.
an. amorphous. silicon. layer. (to. generate. optically. induced. DEP),. and. a. thin. nitride. layer. (to. prevent. potential.
electrolysis)..(b).The.simulation.on.the.electric.ield.distribution.in.the.liquid.layer.of.OET.under.the.illumina-
tion.of.a.Gaussian.beam.with.a.spot.diameter.of.17.μm..(Panels.(a.and.b):.Reproduced.from.Chiou,.P.Y.,.Massively.
parallel.optical.manipulation.of.single.cells,.micro-.and.nano-particles.on.optoelectronic.devices,.Dissertation,.
University. of. California. at. Berkeley,. Berkeley,. CA,. 2005.). (c). The. trapping. of. 20. polystyrene. particles. in. a. 4.×.5.
rectangular.trapping.array,.one.in.each.trapping.spot..(d).The.rearrangement.of.two.different.sizes.of.particles.
in. the. trapping. array.. (Panels. (c. and. d):. Reproduced. with. permission. from. Ohta,. A.T.,. Chiou,. P.Y.,. Han,. T.H.,.
Liao,.J.C.,.Bhardwaj,.U.,.Mccabe,.E.R.B.,.Yu,.F.,.Sun,.R.,.and.Wu,.M.C.,.Dynamic.cell.and.microparticle.control.via.
optoelectronic.tweezers,.
J. Microelectromech Syst
,.16,.491-499,.Copyright.2007,.IEEE.).(e).The.OET.particle.sorting.
design.and.the.fractionation.results.of.a.mixture.of.polystyrene.particles.with.two.different.sizes.of.10.and.24.μm..
(f).The.selective.collection.of.live.cells.from.a.mixture.of.live.and.dead.cells.by.OET..(Panels.(e.and.f):.Reproduced.
by.permission.from.Macmillan.Publishers.Ltd..
Nature
,.Chiou,.P.Y.,.Ohta,.A.T.,.and.Wu,.M.C.,.Massively.parallel.
manipulation.of.single.cells.and.microparticles.using.optical.images,.436,.370-372,.Copyright.2005.)
