DNA Nanostructures - The Nanobiotechnology - page 21

140 nm

90 nm

110 nm 200 nm

(a) (b)

Figure 1.11
(See companion CD for color igure.) .Large.DNA.origami/tile.hybrid.structures..(a).nearly.perfect.assembly.
in.90.×.110 nm.array.(b).multiple.defects.in.140.×.200.nm.array..(Zhao,.Z..et.al.,.A.route.to.scale.up.DNA.origami.
using.DNA.tiles.as.folding.staples.. Angew. Chem ..2010..49..1414-1417..Copyright.Wiley-VCH.Verlag.GmbH.&.Co..
KGaA..Reprinted.with.permission.)

90° 90°

20 nm

Figure 1.12
Predicted.structure.of.“2D”.origami.rectangle..(Adapted.from.Kim,.D.-N..et.al.,. Nucl. Acids Res .,.advanced.access.
DOI:.10.1093/nar/gkr1173,.2011a.under.terms.of.open.access.agreement.)

(Li.et.al..2010,.Woo.and.Rothemund.2011)..In.one.approach,.the.origami.was.stiffened.by.
introducing.a.zigzag.topography.for.the.sheet.of.DNA.helices.that.make.up.the.origami..
Basically,.every.other.helix.was.raised.up.by.a.little.less.than.a.nanometer,.with.the.struc-
ture.enforced.by.the.locations.of.crossover.sites.programmed.by.the.staple.strands..Efforts.
to.construct.2D.arrays.by.connecting.the.corners.of.these.origami.instead.produced.long.
1D.ribbons.or.nanotubes..Woo.and.Rothemund.corrected.the.global.curvature.of.the.2D.
rectangle.by.adjusting.the.crossover.sites.to.relieve.torsional.strains,.and.used.the.resulting.