Biomedical Engineering Reference
In-Depth Information
MFM probe
x
4
3
2
6
2nd trace, phase
2
1
1
5
3
Lift
height
(A)
z
1st trace, Height
z
x
(D)
1st retrace, Amplitude
(B)
Lift height: 0 nm
24
20
18
12
8
4
0
10 40 70 100
Selected distance (nm)
100 nm
120 150
100 nm
Lift height: 0 nm
(b)
Native
(a)
(C)
Native
Lift height: 20 nm
(c)
DTF
(E)
DTF
30
25
20
m
c
= 3. 7
×
10
-17
A
.
m
2
Native
12
10
8
6
4
15
Unstretched
Stretched
10
5
2
0
0
10
20
30
Lift height/nm
40
50
(e)
(d)
Particle size (nm)
Figure 16.2
(See companion CD for color igure.)
.AFM.enables.both.qualitative.and.QI.at.the.nanoscale.level.via.lateral.
movement.of.the.AFM.probe.in.static.or.dynamic.modes..(A).Schematic.of.the.lateral.motion.of.the.AFM.probe.
in.both.static.(a).and.dynamic.(b).modes..(B).Single-molecule.imaging.of.the.globular.protein.DDR2.binding.
to.ilaments.of.collagen.type.I..(Reprinted.with.permission.from.Agarwal,.G.,.Kovak,.L.,.and.Radziejewski,.C.,.
Binding.of.discoidin.domain.receptor.2.to.collagen.I:.An.atomic.force.microscopy.investigation,.
Biochemistry
,.
41(37),.11091-11098..Copyright.2002,.American.Chemical.Society.).(C).AFM.images.of.chondrocytes.before.and.
after.being.subjected.to.dynamic.tensile.stress.(DTF)..(Reprinted.from.
J. Struct. Biol.,
.162(3),.Iscru,.D.F.,.Anghelina,.
M.,.Agarwal,.S..et.al.,.Changes.in.surface.topologies.of.chondrocytes.subjected.to.mechanical.forces:.An.AFM.
analysis,. 397-403,. Copyright. 2008,. Elsevier.). (D). Schematic. of. the. AFM. probe. motion. for. MFM. imaging.. (E).
Height.and.phase.MFM.images.as.well.as.quantitative.phase.shift.values.for.superparamagnetic.nanoparticles..
(Reprinted.from.Schreiber,.S.,.Savla,.M.,.Pelekhov,.D..et.al.:.Magnetic.force.microscopy.of.superparamagnetic.
nanoparticles..
Small
..2008..4(2)..270-278..Copyright.Wiley-VCH.Verlag.GmbH.&.Co..KGaA.)
