Biomedical Engineering Reference
In-Depth Information
16
Atomic Fo
rce Microscopy
Gunjan Agarwal and Tanya M. Nocera
CONTENTS
16.1. Introduction......................................................................................................................... 369
16.2. Introduction.to.AFM.......................................................................................................... 370
16.3. AFM.as.an.Imaging.Tool................................................................................................... 371
16.3.1. Single-Molecule.Imaging...................................................................................... 373
16.3.2. Cell.and.Tissue.Imaging........................................................................................ 374
16.3.3. Imaging.the.Interior.of.Samples........................................................................... 375
16.4. AFM.as.a.Force.Sensor....................................................................................................... 376
16.4.1. Molecular.and.Cellular.Adhesion.and.Recognition......................................... 376
16.4.2. Single-Molecule.Mechanics.................................................................................. 378
16.4.3. Cell.Mechanics.and.Rheometry........................................................................... 379
16.4.4. Micromechanics.of.Single.Fibers......................................................................... 379
16.5. AFM.as.a.Nanomanipulator............................................................................................. 380
16.5.1. Manipulation.of.Nanoparticles............................................................................ 380
16.5.2. Dip-Pen.Nanolithography..................................................................................... 382
16.5.3. Nanografting........................................................................................................... 383
16.6. Recent.Developments.in.AFM.......................................................................................... 384
16.7. Conclusions.and.Future.Perspectives.............................................................................. 385
Acknowledgments....................................................................................................................... 385
References..................................................................................................................................... 386
16.1 Introduction
The. aim. of. this. chapter. is. to. provide. an. overview. of. the. applications. of. atomic. force.
microscopy.(AFM).in.nanobiotechnology..We.avoid.details.on.the.operational.theory.of.or.
sample.preparation.for.AFM,.but.instead.direct.the.reader.to.several.excellent.texts.(cited.
in.the.references).on.these.topics..This.chapter.serves.as.a.user's.guide.to.the.applications.
of. AFM. in. nanobiotechnology. without. unnecessary. repetition. of. previously. published.
work.and.without.mathematical.detail..We.compare.and.contrast.the.capabilities.of.AFM.
with.other.microscopic.techniques.and.introduce.the.reader.to.standard.capabilities.of.the.
AFM.relevant.for.molecular.and.cellular.detection.and.manipulation..Finally,.we.highlight.
the.recent.developments.and.upcoming.techniques.involving.AFM.for.nanobiotechnology.
applications.and.conclude.with.limitations.and.future.trends.of.the.AFM.
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