Biomedical Engineering Reference
In-Depth Information
50-100 µm
1
PDMS
PDMS
2
3
Glass
4
50-100 µm
Glass
Microarray of secreted products
Figure 15.8
Schematic.diagram.depicting.method.for.preparation.of.engraved.arrays.of.secreted.products.from.a.mixture.
of.cells..(1).A.suspension.of.cells.is.deposited.onto.an.array.of.microwells.fabricated.by.soft.lithography..(2).The.
cells.are.allowed.to.settle.into.the.wells.and.then.the.excess.medium.is.removed.by.aspiration..(3).The.dewetted.
array.is.placed.in.contact.with.a.pretreated.solid.support,.compressed.lightly,.and.incubated.for.2-4.h..(4).The.
microwells.are.removed.from.the.solid.support.and.placed.in.a.reservoir.of.medium..(Reprinted.by.permission.
from.Macmillan.Publishers.Ltd..
Nat. Biotechnol
.,.Love,.J.C.,.Ronan,.J.L.,.Grotenbreg,.G.M.,.Van.Der.Veen,.A.G.,.
and.Ploegh,.H.L.,.A.microengraving.method.for.rapid.selection.of.single.cells.producing.antigen-speciic.anti-
bodies,.24,.703-707,.copyright.2006.)
After.ethanol.sterilization,.the.chips.were.able.to.support.the.growth.of.a.Gram-positive.
bacterium.(
L. plantarum
.WCFS1),.a.Gram-negative.bacterium.(
E. coli
.XL2.Blue),.and.a.yeast.
(
C. albicans
.JBZ32)..They.were.also.able.to.separate.and.isolate.luorescent.organisms.from.a.
nonluorescent.control.of.the.same.organism..A.subsequent.review.by.this.group.(Ingham.
et.al..2011),.discusses.a.number.of.other.applications.of.PAO.including.investigating.the.
communication. and. competition. between. different. strains. of. the. swarming. bacterium.
Proteus mirabilis
.and.studying.communication.between.malarially.infected.and.uninfected.
human.blood.cell.populations.
While.the.majority.of.3D.culture.devices.are.targeted.at.tissue.engineering,.a.few.have.
more. general. applicability.. Anada. et. al.. (2010). recently. reported. a. novel. multicellular.
aggregate. (spheroid). culture. device. that. utilizes. thin. polydimethylsiloxane. membrane.
