Biomedical Engineering Reference
In-Depth Information
E
E
NH
2
H
2
N
TEOS
1) Glutaraldehyde
TEOS
O
O
O
O
O
O
O
O
APTS/PEOS
2) Enzymes/NaBH
2
CN
γ
-Fe
2
O
3
NH
2
E
γ
-Fe
2
O
3
-O SiO
2
H
2
N
E
γ
-Fe
2
O
3
O SiO
2
-NH
2
/PEG
γ
-Fe
2
O
3
O SiO
2
-enzyme/PEG
E
Enzymes
Figure 15.6
Silica. core-shell. nanoparticle. synthesis. and. enzymes. anchoring.. (With. kind. permission. from. Springer.
Science+Business.Media:.
J. Nanopart. Res
.,.Design.of.multifunctionalized.c-Fe
2
O
3
@SiO
2
.core-shell.nanoparticles.
for. enzymes. immobilization,. 12,. 2009,. 675-680,. Georgelin,. T.,. Maurice,. V.,. Malezieux,. B.,. Siaugue,. J.M.,. and.
Cabuil,.V.,.Figure.1,.Copyright.2009.)
In.another.application.of.Ni
2+
.interactions.with.His-tagged.proteins,.Wang.et.al..(2011).
synthesized.oleic.acid-coated.iron.oxide.nanoparticles..Glycidyl.methacrylate.(GMA).was.
then. polymerized. in. the. presence. of. these. OA-MNPs. creating. the. core-shell. structured.
nanoparticles.containing.an.epoxy.function.at.the.surface.(PGMA-MNPs)..Further.reaction.
with.ethylenediamine.gave.the.corresponding.MNPs.with.amino.group.(EDA-MNPs),.and.
then. an. aldehyde. group. was. introduced. by. subsequent. reaction. with. glutaraldehyde. to.
give.GA-MNPs.(Figure.15.7)..An.amine-containing.NTA.derivative.was.reacted.with.the.
O
O
O
OH
-
O
Fe
Z+
+ Fe
3+
+HO
O
OA-MNPs
PGMA-MNPs
O
H
2
N
NH
2
O
O
HO
H
2
N
N
O
O
OH
O
HO
R
2
H
R
1
R
2
OH
H
N
H
NH
2
O
OH
O
OH
O
NTA-MNPs
GA-MnPs
EDA-MnPs
O
His-tag
.......
enzyme
O
O
O
Cell
free
extract
.......
O
O
His-tag
Other proteins
enzyme
O
O
O
NH
R
y
R
3
N
N
2+
NP
CH
2
CH
2
O
NH
N
O
O
O
R
x
N
O
Ni-NTA-MNPs
R
z
N
H
NH
HN
H
21EH1-Ni-NTA-MNPs
OH
OH
N
N
R
y
H
OH
Figure 15.7
Synthesis. of. Ni-NTA-MNPs. and. their. application. for. puriication. and. immobilization. of. His-tagged. enzyme.
in.one.pot.
