Biomedical Engineering Reference
In-Depth Information
O
HN
NH
Az
N
O
N 3
S
O
O
O
O
O
HN
NH
Biotin
Biotin
N 3
Biotin
O N
Es
O
1.
1.
1.
O N
O
(Az)
Cu(I), THTA
S
(Al)
Cu(I), THTA
O
O
(Es)
O
O
HN
NH
Al
O
2.
2.
2.
N
N
(b)
S
(a)
O
(c)
(d)
(e)
(i)
(j)
(f)
(g)
(h)
249 counted
84% yield
197 counted
90% yield
227 counted
84% yield
180 counted
69% yield
Figure 1.4
(See companion CD for color igure.) .Chemical.conjugation.reactions.applied.to.functional.groups.displayed.
on. DNA. origami.. Reactions. were. done. on. origami. supported. on. mica. surfaces.. (a). DNA. origami. derivatized.
with.an.azide,.primary.amine,.terminal.alkyne,.and.biotin.groups..(b).Structures.of.the.reaction.partners.used..
(c/f).“Click”.reaction.on.terminal.alkyne.groups..(d/g).Amide.formation.at.primary.amine.groups..(e/h).“Click”.
reaction. on. azide. groups.. (i/j). All. three. reactions. done. in. series.. (Reprinted. by. permission. from. Macmillan.
Publishers.Ltd.. Nat. Nanotechnol .,.Voigt,.N.V.,.Tørring,.T.,.Rotaru,.A.,.Jacobsen,.M.F.,.Ravnsbæk,.J.B.,.Subramanil,.R.,.
Mamdouh,.W.,.Kjems,.J.,.Mokhir,.A.,.Besenbacher,.F.,.and.Gothelf,.K.V.,.Single-molecule.chemical.reactions.on.
DNA.origami,.5,.200-203,.Copyright.2010.)
on. a. surface. can. survive. exposure. to. organic. solvents. such. as. tetrahydrofuran. (THF).
without.unbinding,.structural.degradation,.or.aggregation.
1.6.2  Computer Modeling and Design Tools
The. breakthroughs. in. origami. design. have. centered. around. improved. structural.
understanding. and. design. tools.. CAdnano. (Douglas. et. al.. 2009a,b,.Ke. et. al.. 2009a). is. an.
excellent.design.tool.for.both.2D-.and.3D.DNA.origami,.available.as.a.free.and.open.source.
download.(http://cadnano.org/);.see.Figures.1.5.and.1.6..Other.origami.design.programs.
are.available,.such.as.SARSE.(Andersen.et.al..2008,.http://cdna.au.dk/software/).
1.6.3  3D DNA Nanostructures
The.original.designs.for.DNA.origami.produced.relatively.large,.lat.sheets.of.material..Like.
a.sheet.of.paper,.a.lat.origami.can.be.folded.or.rolled.to.create.many.types.of.3D.structures..
By.mapping.the.four.triangular.faces.of.a.tetrahedron.onto.a.2D.strip.of.DNA.origami,.with.
“crease.marks”.deined.by.short.regions.of.unstructured.DNA,.Ke.et.al..(2009b).designed.
 
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