Biomedical Engineering Reference
In-Depth Information
TABLe 10.1
Examples.of.Olfactory.Receptors.Used.in.Cell-Free.Biosensor.Platforms
Analytes Tested (Smell
Characteristic), [Lowest
Concentration Tested]
Olfactory
Receptor
Sensing
Platform
Expression System and
Immobilization Phase
Immobilization Technique
References
Rat.OR-I7
EIS
Octanal.(citrus),.heptanal.
(green.vegetables),.
helional.(sweet.harsh.hay,.
taste.of.metal).[10 −11 .M]
Membrane.fraction.from.
Saccharomyces cerevisiae
MHDA.+biotinyl-PE.SAM
Hou.et.al..(2007).and.
Alinito.et.al..(2010)
SPR
Octanal
Nanosomes.from. S. cerevisiae
Dextran.coated
Vidic.et.al..(2006)
Mouse.OR174-9
FET.(CNT)
Eugenol.(cloves)
Acetophenone.(fruity)
Methyl.benzoate
Cyclohexanone
Sf9.insect.cell.expression,.
his-tag.puriication,.micelle,.
or.nanodisc.immobilization
Histag-Ni-NTA.anchoring
Goldsmith.et.al..
(2011)
Mouse.OR203-1
FET.(CNT)
2-Heptanone.(banana)
Sf9.insect.cell.expression,.
his-tag.puriication,.micelle,.
or.nanodisc.immobilization
Histag-Ni-NTA.anchoring
Goldsmith.et.al..
(2011)
Mouse.OR256-17
FET.(CNT)
2-Heptanone,.heptanal,.
acetophenone,.2,4.DNT.
n -amylacetate.methyl.
benzoate,.cyclohexanone
Sf9.insect.cell.expression,.
his-tag.puriication,.micelle,.
or.nanodisc.immobilization
Histag-Ni-NTA.anchoring
Goldsmith.et.al..
(2011)
Human.OR1740
SPR
Helional,.cassione.(sweet.
loral)
Nanosomes.from. S. cerevisiae
Dextran.coated
Vidic.et.al..(2006)
SPR
Helional
Nanosomes.from. S. cerevisiae
SAMs:.BATs.with.PEG-terminated.
alkanethiols.(PEGATs);.MHDA.
±biotinyl-PE;.OR.Myc-tag.antibody.
capture
Vidic.et.al..(2007)
Human.OR17-4
SPR
Lilial.(lily.of.the.valley),.
loralozone.[10 −6 .M]
Detergent-solubilized.
puriied.protein.from.
HEK293.inducible.
expression.system
OR.Rho-tag.antibody.capture
Cook.et.al..(2009)
SPR
Undecanal.(loral,.waxy,.
citrus).[10 −6 .M]
Cell-free.wheat.germ.lysate.
expression
Anti-His.antibody.capture
Kaiser.et.al..(2008)
( continued )
 
Search WWH ::




Custom Search