Biomedical Engineering Reference
In-Depth Information
10 μm
10 μm
(a)
(b)
Channel axis
(c)
(d)
Figure 8.7
(See companion CD for color igure.)
. Shape. memory. biomaterials. hold. great. promise. for. future. studies. of.
topographical.cues..In.this.particular.study,.poly(ε-caprolactone).substrates.underwent.(a).thermal.transforma-
tion.and.(b).recovery..GFP-tagged.hMSCs.exhibited.morphological.changes.in.response.to.such.treatment..These.
cells.aligned.with.the.3.μm.×.5.μm.channels.(c),.but.upon.return.to.the.original.planar.surface,.they.adopted.an.
unaligned. orientation. (d). (scale. bars. for. c,. d:. 100.μm).. These. preliminary. indings. highlight. the. feasibility. of.
dynamic. control. over. surface. topographical. features.. (Le,. D.M.,. Kulangara,. K.. et. al.:. Dynamic. topographical.
control. of. mesenchymal. stem. cells. by. culture. on. responsive. poly(epsilon-caprolactone). surfaces..
Adv. Mater
..
2011..23(29)..3278-3283..Copyright.Wiley-VCH.Verlag.GmbH.&.Co..Reprinted.with.permission.)
8.5 Summary
By.more.appropriately.embodying.the.native.ECM.environment,.nanotopographical.fea-
tures. created. by. either. physical. or. chemical. means,. or. through. a. combination. of. both,.
have. effectively. elicited. desirable. cell. responses.. Various. cell. types. react. differently. to. a.
given.cue,.and.the.mechanisms.behind.these.manifestations.must.be.better.comprehended.
to. advance. the. ield..Achieving. optimal. results. for. the. intended. function. requires. rein-
ing.of.the.current.nanofabrication.techniques,.inding.the.most.suitable.biomaterials,.and.
determining.the.appropriate.nanopattern.shape.and.spacing..As.the.driving.forces.behind.
cell.adhesion,.proliferation,.and.differentiation.continue.to.unfold,.biomimetic.materials.
undoubtedly.hold.tremendous.prospects.for.tissue.engineering.and.regenerative.medicine.
applications..ECM-inspired.scaffolds.will.likely.serve.as.a.versatile.and.potent.platform.to.
cultivate.organ.and.tissue.development.for.therapeutic.beneit.
Acknowledgment
The.authors.would.like.to.thank.NIH.for.funding.support.(R21EB015300).
