Biomedical Engineering Reference
In-Depth Information
5 µm
(a)
(b)
10 µm
10 µm
(c)
(d)
000035
10 KV ×
2 5 0
1 2 0 um
.
000035
10 KV 1 30 K 23 1um
×
.
(e)
(f)
Figure 8.6
(See companion CD for color igure.)
.Morphological.differences.become.evident.with.nanoscale.surface.alter-
ation.. (a,. b). Epithelial. cells. cultured. on. planar. silicon. oxide. display. a. rounded. shape,. whereas. those. on. the.
nanopatterned.surface.(70.nm.ridges,.400.nm.pitch,.600.nm.groove.depth).were.elongated.and.aligned.with.the.
nanogrooves.. (Reprinted. with. permission. from. Teixeira,. A.I.. et. al.,.
J. Cell Sci
.,. 116(10),. 1881,. 2003.. Copyright.
The.Company.of.Biologists.Ltd.).(c,.d).Human.fetal.osteoblasts.situated.on.islands.of.height.11.nm.experienced.
greater. spreading. and. distinct. lamellopodia. compared. to. those. on. lat. polystyrene.. (Lim,. J.Y.,. Hansen,. J.C.,.
Siedlecki,.C.A.,.Runt,.J.,.and.Donahue,.H.J.,.Human.foetal.osteoblastic.cell.response.to.polymer-demixed.nano-
topographic. interfaces,.
J. R. Soc. Interface
,. 2(2),. 97-108,. 2005.. Reprinted. by. permission. of. The. Royal. Society. of.
Publishing.).(e,.f).Fibroblasts.(hTERT-BJ1).cultured.on.surfaces.bearing.120.nm.diameter.nanopits.demonstrated.
less. cell. spreading. than. cells. on. the. smooth. control. substrate.. (Dalby,. M.J.,. N.. Gadegaard,. M.O.. Riehle. et. al..
2004a..Investigating...ilopodia.sensing.using.arrays.of.deined.nano-pits.down.to.35.nm.diameter.in.size..
The
International Journal of Biochemistry and Cell Biology
.36(10):.2005-2015..Reprinted.with.permission.from.Elsevier.)
