Biomedical Engineering Reference
In-Depth Information
Overall modification strategy:
p
aF19
1.
2.
Isolate assembled capsids from
E
.
coli
expression
Modification of internal Cys 87 with maleimide 1
27 nm
3.
Attachment of DNA aptamers to external
p
aF
residues through oxidative coupling
Cys 87
MS2 coat protein dimer
Internal modification:
SO
3
H
Targeted carrier containing
up to 180 porphyrins
External modification:
O
NH
N
Et
2
N
N
HO
3
S
O
O
O
O
O
O
O
N
HN
O
O
P
P
Aptamer
O
Aptamer
HN
H
H
H
O
3
5
3
5
O
O
NH
2
N
5 mM NalO
4
Phosphate bu
er
pH 7, 150 mM NaCl
RT, 1h
O
NEt
2
MS2
p
aF19
SO
3
H
Porphyrin maleimide 1
Figure 7.8
(See companion CD for color igure.)
.Interior.and.exterior.modiications.of.the.MS2.bacteriophage.for.use.in.targeted.photodynamic.therapy..(Reprinted.with.per-
mission.from.Stephanopoulos,.N.,.Tong,.G.J.,.Hsiao,.S.C.,.Francis,.M.B.,.Dual-surface.modiied.virus.capsids.for.targeted.delivery.of.photodynamic.agents.to.cancer.
cells,.
ACS Nano
,.4(10),.6014-6020..Copyright.2010b.American.Chemical.Society.)