Biomedical Engineering Reference
In-Depth Information
Overall modification strategy:
p aF19
1.
2.
Isolate assembled capsids from E . coli expression
Modification of internal Cys 87 with maleimide 1
27 nm
3.
Attachment of DNA aptamers to external p aF
residues through oxidative coupling
Cys 87
MS2 coat protein dimer
Internal modification:
SO 3 H
Targeted carrier containing
up to 180 porphyrins
External modification:
O
NH
N
Et 2 N
N
HO 3 S
O
O
O
O
O
O
O
N
HN
O
O
P
P
Aptamer
O
Aptamer
HN
H
H
H
O
3
5
3
5
O
O
NH 2
N
5 mM NalO 4
Phosphate bu er
pH 7, 150 mM NaCl
RT, 1h
O
NEt 2
MS2 p aF19
SO 3 H
Porphyrin maleimide 1
Figure 7.8
(See companion CD for color igure.) .Interior.and.exterior.modiications.of.the.MS2.bacteriophage.for.use.in.targeted.photodynamic.therapy..(Reprinted.with.per-
mission.from.Stephanopoulos,.N.,.Tong,.G.J.,.Hsiao,.S.C.,.Francis,.M.B.,.Dual-surface.modiied.virus.capsids.for.targeted.delivery.of.photodynamic.agents.to.cancer.
cells,. ACS Nano ,.4(10),.6014-6020..Copyright.2010b.American.Chemical.Society.)
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