Biomedical Engineering Reference
In-Depth Information
TABLe 4.1 (continued)
Applications.of.Biomolecular.Motors.in.Nanotechnology
Motor
Guidance
Experiment
Future Function/Application
Viability
Reference
Recombinant.
kinesin.
K401-bio.(401.
AA.of.the.
N-terminal.
motor.domain.
of.the.heavy.
chain.of.
D. melanogaster
.
Kin-1).linked.
to.BCCP.
sequence.of.
E. coli
Glide;.docked.kinesin.
motors
Organization.of.interlinked.
asters.polymerized.over.an.
absorbed.and.functionalized.
kinesin.motor.surface
Deposition.of.mitotic.
spindle-like.structures.to.be.
used.and.synthesized.at.the.
nanoscale.from.the.
bottom.up
Complex.tubulin.aster.
structures.have.been.imaged;.
constitutes.a.progression.
toward.mimicking.mitotic.
mechanisms
Nedelec.et.al..
(1997);.Surrey.
et.al..(2001)
Kinesin
Surface-bound.MTs.
driven.autonomously.
by.kinesin.clusters.
mimic.beating.of.cilia
Streptavidin.molecules.
bound.to.four.biotinylated.
kinesins.each.allow.kinesin.
clusters.to.bundle.MTs;.
ATP-dependent.kinesin.
clusters.spontaneously.
stimulate.cilia-like.
dynamics
Powering.mechanism.for.
motility.of.larger.complexes.
and.vessels;.biomimicry.of.
axonal.structures.with.a.
possible.functional.
replacement.of.dynein
Cilia-like.beating.through.
kinesin-driven.MT.bundles.
experimentally.demonstrated;.
mechanism.not.totally.
understood;.integration.into.
device.is.far.off
Sanchez.et.al..
(2011)
Kinesin
Glide;.docked.kinesin.
motors;.docked.single.
stranded.30.nt.DNA;.
MT.shuttles.
functionalized.with.
small.DNA.“zippers”.
of.varying.strength
MT.shuttles.over.loading-
transport-unloading.
regions;.cargos.immobilized.
by.30.nt.overlap.DNA.
zippers;.binds.to.MT.at.
loading.station;.cargo.binds.
to.unloading.station.after.
transport.by.shearing.
geometry.of.30.nt.regions;.
weaker.20.nt.MT-cargo.
attachment.breaks.and.MT.
moves.on.without.cargo
Loading.and.unloading.of.
cargo.for.lab-on-a-chip.
devices
Currently.a.candidate.for.
solving.the.loading/unloading.
problem.of.cargo.transport;.
rupture.forces.experimentally.
shown;.low.eficiency.due.to.
low.collision.probability;.
functional.mechanism.remains.
in.its.infancy
Schmidt.and.
Vogel.(2010).
(see.review)
