Biomedical Engineering Reference
In-Depth Information
Fig. 12 CNTs act as a vector for drug delivery into living cells. After incubation of HeLa cells
with AlexaFluor594-labeled SWNTs for 12 h at 37
C, living cells were observed under confocal
fluorescence microscope for a CNT uptake study. (a) Images show dual confocal detection of
AlexaFluor594-SWNT (
red
) internalized into cells with the membrane stained by AlexaFluor488
(
green
). (b) Series of images of different
z
-focal scanning planes down through cells. (Adapted
from [
61
])
Another method for the synthesis of stable metal nanoparticles involves first
mixing the metal hydrosols and an ethanol solution of dodecylamine and then
extracting the dodecylamine-stabilized metal nanoparticles into toluene. The etha-
nol, a water miscible and good solvent for dodecylamine, was used as an interme-
diate solvent to improve the interfacial contact between citrate-stabilized metal
nanoparticles and alkylamine. The extraction of dodecylamine-stabilized metal