Biomedical Engineering Reference
In-Depth Information
Fig. 13 Phase contrast microscope images of mouse embryonic stem cells in the PMBV/PVA
hydrogel (
left
) and on TCPS (
right
)
Fig. 14 Alkaline phosphatase staining of recovered ES cells after dissociation of PMBV/PVA
hydrogel (
left
), and of the ES cells cultured on PMB30 (
right
). The undifferentiated ES cells were
well stained. ES cells encapsulated in the PMBV/PVA hydrogel maintained their undifferentiated
character during the 3 days of encapsulation
by ALP staining. Indeed, this is an indicator of the differentiation of ES cells. It
should be noted that the colonies were also well stained by ALP staining. This result
indicated that the ES cells maintained their undifferentiated character during the
immobilization period in the PMBV/PVA hydrogel. It can be seen that the PMBV/
PVA hydrogel supported the cell immobilization space in their network without any
stimulation to change the cell function.
8 Cell-Based Biochips Fabricated Using a PMBV/PVA
Hydrogel
8.1 Microfluidics for Miniaturized Cell Operation
Microfluidics evolved from micro-analytical methods in capillary format such
as capillary electrophoresis, high-performance liquid chromatography, and gas
chromatography, and has successfully revolutionized chemical and biochemical
