Biomedical Engineering Reference
In-Depth Information
Fig. 10 Cell cycle
distribution of fibroblast
(L929) cells depends
on the elapsed time after
encapsulated in the PMBV/
PVA hydrogel and on culture
time on TCPS
0 h
1 day
2 days
3 days
in cooled 70% ethanol for 2 h. The cells were incubated in a solution containing
1 mg/mL RNase and 20 mg/mL propidium iodide (PI) for 30 min. Cell cycle
analysis was performed by flow cytometry.
Figure
10
shows the distribution of cell cycle phases of L929 cells encapsulated
in the PMBV/PVA hydrogel and those cultured on the TCPS. The percentage of
cells in G0/G1 phase was 71% in the PMBV/PVA hydrogel after 3 days. By
contrast, the amounts in G0/G1 phase were 30-50% in conventional cultivation
on the TCPS during the 3 days. This means that L929 cells divided and proliferated
more uniformly in the PMBV/PVA hydrogel than on the TCPS. As a result, the
distribution of cell cycle phases of L929 cells cultured in the PMBV/PVA hydrogel
was more uniform than that on the TCPS. In the PMBV/PVA hydrogel, 94% of
L929 cells were synchronized to G0/G1 phase at 7 days. On the TCPS, 97% of L929
cells were also synchronized to G0/G1 phase at 7 days. However, the cell numbers
had been decreasing from 4 days so that L929 cells had entered into the death phase
after 7 days on TCPS. L929 cells became confluent and cell death was induced.
5 Cell Functions in the PMBV/PVA Hydrogel
Liver cells, HepG2, could also be encapsulated in the PMBV/PVA hydrogel, and
the cell-specific functions were evaluated. HepG2 cells secrete albumin during cell
culture. Culture supernatants were collected and albumin content measured using
