Cationic Polymers for the Delivery of Therapeutic Nucleotides - Nanotechnology for the Delivery of Therapeutic Nucleic Acids - page 43

into mammalian cell using PHP ester-DNA complexes showed the
possibility of using PHP ester as a biodegradable gene delivery
carrier.

78, 79

synthesized biodegradable cationic polymer,
poly[alpha-(4-aminobutyl)-L-glycolic acid] (PAGA). Because
of degradability and the biocompatibility of the monomer, this
polymer does not showed any cytotoxicity. Furthermore, PAGA-DNA
complexes exhibited higher transfection efficiency in cells and were
reported as useful gene delivery agents.

Lim et al.

80

Li and Huang synthesized and characterized copolymer
poly(D,L-lactide-co-4-hydroxy-L-proline) (PLHP) as a polymeric
gene carrier for sustained gene delivery. The pDNA-loaded
microspheres based on the copolymer were prepared by solvent
evaporation emulsion method. The release profile of pDNA from
PLHP microspheres showed an initial burst and then slower and
continuous release for about 18 days. Gene transfer efficiency of
PLHP/pDNA delivery system showed a sustained activity (over a
week) when compared with PEI and PLL, suggesting that PLHP
is a promising candidate for long-term gene delivery with good
biocompatibility and biodegradability.

81

describes the synthesis of nanosized particles
consisting of PEI (25 kDa) crosslinked with PCL (50-60 kDa).
The nanoparticles obtained were characterized in relation to
their physicochemical and biological properties, and results
are extremely promising in terms of low cell toxicity and high
transfection efficiency.

d'Ayala et al.

82

Further, to overcome toxic effects of
PEI with a minimal impact on transfection efficiency, non-ionic
biocompatible polyesters have been conjugated with PEI. Series of
PEI and γ-PGA nanocomposites were prepared and evaluated in
terms of cell viability and transfection efficiency by Tripathi et al.

Results obtained demonstrated the potential of the nanocomposites
for gene delivery.

34

Biodegradable PLA and PLGA microsphere/nanoparticles

are advance thriving approaches for nucleotide delivery;

due to small particles size, they are easy to inject directly to the
targeted organs.

in vivo

83, 84

reported biodegradable cationic
nanoparticles (cNP) made of PLA as a promising carrier for

Munier et al.

in

vivo DNA delivery and immunization. A versatile approach for the

use of pre-formed particles and subsequent adsorption of a model
polycation (PEI) was described. Two more polycations, chitosan and
poly(2-dimethyl-amino) ethyl methacrylate (pDMAEMA) were also