Biomedical Engineering Reference
In-Depth Information
23
cell or tissue.
Owing to high transfection efficiency, branched PEI
with a molecular weight (MW) of 25 kDa is considered to be the
“gold standard” for polymeric non-viral vectors.
24
High transfection
efficiency of PEI, along with its cytotoxicity, strongly depends on its
MW.
OH
Chitosan
OH
O
O
OH
O
OH
HO
O
NH
2
O
HO
PEI
NH
O
O
O
HO
O
NH
2
HO
n
NH
2
O
O
H
HO
Dextran
O
OH
O
H
HO
O
OH
PLL
O
H
HO
O
OH
O
n
O
H
HO
O
HO
O
D-SPM
HO
HN
OH
n
NH
NH
NH
2
Cyclodextrin
Figure 2.2
Chemical structure of cationic polymers for the delivery of
nucleotides.
To enhance its gene delivery efficiency and minimize cytotoxi-
city, small cross-linked PEI with biodegradable linkages were
synthesized and evaluated for their transfection efficiencies
in
25
vitro
reported a simple, reproducible process to
condense pDNA with PEI. PEI-DNA complexes with 30-60 nm
diameter and stable for more than one month at 4°C with respect
to size and transfection activity were prepared. Intraperitoneal
administration of these PEI-condensed DNA resulted in significant
gene expression in a human ovarian cancer peritoneal xenograft
model.
.
Chen
et al.
reported a simple three blending methods
to combine biodegradable polyurethane and PEI with DNA by
unique mixing sequences. The complex prepared showed better
26
Cherng
et al.
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