Lipoplexes and Polyplexes: From Gene Delivery to Gene Expression - Nanotechnology for the Delivery of Therapeutic Nucleic Acids - page 17

H

NH 2

H

N O

O

NH 2

H

H

NH 2

O N

NH

O

NH 2

S N O O

O

O

N S

O

H

N NH 2

O
H

H

H

N H

H

NH 2 H

NH 2

O

NH 2

H

O

Figure 1.1 Structure of reduction-sensitive biodegradable dendrimer

FIM-2126.

The products were mixed with DNA at different charge ratios
and were run on gel electrophoresis in the presence or absence of
dithiothreitol. Figure 1.18 shows that the products retain the DNA
at positive charge ratios similarly to non-reducible polycations.
On the other side, incubation of the complexes with DTT disrupts
completely the complexes, and DNA runs freely independently of the
charge ratios.

Figure 1.1 Gel electrophoresis obtained for DNA complexes of the

polypeptides in absence (left panel) or presence of DTT (right
panel). Lanes 1 and 12 stand for uncomplexed DNA.

Figure 1.1 Left panel: Transfection levels in 3T3-BALB/c fibroblasts

for dendrimer FIM-2126/SEAP-DNA complexes at different
charge ratios (+/-). Right panel: XTT viability assay in 3T3-
BALB/c fibroblasts of dendrimer FIM-2126 at different charge
ratios (+/-).