Biomedical Engineering Reference
In-Depth Information
In further works [12], we have used compound RPR-120535
in vivo
for correcting anemia in a uremic-mice model by gene delivery
and expression of erythropoietin (EPO) to peritoneal mesothelial
cells that, upon gene delivery, secreted EPO to blood.
Finally, MCO-I-68, a lipopolyamine with a gadolinium complex-
ing arm on its side chain (see Fig. 1.7), has been synthesized as probe
for
distribution studies of DNA/lipopolyamine complexes
using MRI technics [31].
in vivo
N
H
2
O
H
N
H
2
N
H
b
o
c
N
N
2
N
H
N
N
N
H
O
H
N
O
H
2
N
N
1. H
2
, Pd/C, MeOH
N
N
H
N
O
2. DTPA-precursor 1
BOP TEA DCM
3. TFA
O
H
O
b
o
c
O
N
H
H
N
O
N
H
O
H
GdCl
3
.6H
2
O
O
O
N
b
o
c
N
O
H
O
C
2
N
N
Gd
N
N
H
N
O
O
O
O
H
O
O
O
O
O
N
H
O
O
2
N
MCO-I-68
O
H
MCO-I-68-Gd complex
Figure 1.
Synthesis of MCO-I-68 and its complex with gadolinium for
MRI.
Results in panels A and B in Fig. 1.8 show the clear contrast
obtained even after 48 h after administration of two different
formulations of MCO-I-68.
A
B
Figure 1.
MRI Images of mice.
A: 48 h after injection of the left side tumor
with MCO-I-68-Gd/DOPE/ DNA complexes. Right side tumor
is the non-injected tumor control. B: 48 h after injection of the
right side tumor with MCO-I-68-Gd/DNA lipoplex.
1.
Lipopolyamine Co-formulation with DNA
Complexing Peptides
In the second approach, we have devised a system in which DNA
is associated with short peptides derived from human histone or
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