Bioresponsive Nanoparticles for the Intracellular Delivery of RNAi Therapeutics - Nanotechnology for the Delivery of Therapeutic Nucleic Acids - page 134

triggers to engage at different levels of the RNAi cascade and (2)
nanoparticle disassembly to potentiate incorporation into the RNAi
machinery and tune biological activity. Incorporation of functional
components at different molar ratios during the polymerisation
process in these systems allows detailed investigations into the role
and polymer content requirement for optimal intracellular spatial
and temporal RNAi effects.

Figure 6.1 Schematic representation of bioresponsive polymers descri-

bed in this chapter (A) multiblock reducible copolypeptide
(rCPP) system (adapted from reference 56). (B) Reducible
hyperbranched (rHB) system (adapted from reference 55).
In both systems, the ratio of each component can be varied
to study the structural-activity relationship.

6.4 Copolypeptide System

The bioreducible copolypeptide (rCPP) system is based on a
series of polymers composed of different molar ratios of a lysine-