Biomedical Engineering Reference
In-Depth Information
(Fig. 12.2b). After seeding for 5 h, the cells enlarge and latten
obviously (Fig. 12.2c) and are in close contact with the coating via
the extended ilopodia. With further increase in the culturing time to
24 h, all the cells completely latten (Fig. 12.2d). After culturing for
48 h, the cells appear to be more elongated and thicker (Fig. 12.2e).
The results indicate that the MG63 cells can attach and adhere well
to the plasma-sprayed monoclinic zirconia coating. Cell attachment
and adhesion are the irst phase of cell/material interaction and the
eficacy and quality of this irst phase will inluence the capability
of the cells to proliferate and differentiate upon contact with the
implant.
Figure 12.2
SEM pictures of MG63 cells cultured on the plasma-sprayed
monoclinic zirconia coating for (a) 1 h, (b) 2 h, (c) 5 h,
(d) 24 h, and (e) 48 h (the density of the cell suspension is
1 × 10
4
cell ml
-1
) [130].
Figure 12.3 displays the morphology of the MG63 cells seeded on
the coating surface for 3-11 days showing higher cell densities. After
seeding for 3 days, the number and size of cells increase, indicating
